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The Vacc-iNTS training of staff from African countries on serological assays has been held virtually on 5th and 6th November 2020. The theoretical course aimed at training local laboratory staff in Malawi, Burkina Faso, Kenya and Ghana for the seroepidemiological studies and serological testing of the Vacc-iNTS project (activities performed under work package 5, 7 and 10) and involved 29 trainee participants. This training was also held in order to build capacity and support future fields studies at these sites.